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Opcje przeglądania

The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small and large scale.

B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.


High Protein Yield

As a result, protein yields are exceptionally high even if inexpensive substrates are used. For example, the proteins mutarotase (Mro) and glucose dehydrogenase (Gdh) have been accumulated up to 20% and 30% of the total soluble protein, respectively. Using the tightly regulated xylose operon, the genes were induced 130- to 350-fold without proteolysis.


Versatile System with a Wide Range of Vectors

MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable for most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence, a 6xHis-tag, a Strep-tag, and a Strep/6xHis double-tag. Additionally, all vectors contain the tightly regulated and highly inducible xylose operon promoter.

B. megaterium protoplasts specifically optimized for transformation

The protoplasts supplied by MoBiTec are from B. megaterium strain WH320 developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies.
For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.

Features:

  • Stable, high-yield protein expression in Bacillus megaterium
  • Ideal for both small and large-scale protein production
  • Tightly regulated and efficiently inducible xylose operon (up to 350-fold)
  • No alkaline proteases activity even up to 5 hours after induction
  • No endotoxins observed
  • Versatile cloning (extended polylinker, additional BsrG I site*)
  • Versatile production (intracellular or extracellular)
  • Versatile purification (native, 6xHis-tag, Strep-tag, Strep/6xHis double-tag)
  • Removeable tag versions (for TEV or Xa proteases) available
  • Compatible with all B. subtilis vectors

*BsrG I site is not included in all vectors.

Expression Vectors for B.megaterium

Sortuj wg: ▲ Nazwa produktu | Cena
Nazwa produktu / Producent Nr. katalogowy Opakowanie Cena brutto  
Bacillus megaterium protoplast, strain WH320
MoBiTec
BMEG02 5x500 µl
424,84 €
Bacillus megaterium vector pC-His1622
MoBiTec
BMEG20 10 µg
353,38 €
Bacillus megaterium vector pC-Strep1622
MoBiTec
BMEG21 10 µg
434,93 €
Bacillus megaterium vector pN-His-TEV1622
MoBiTec
BMEG22 10 µg
353,38 €
Bacillus megaterium vector pN-Strep-TEV1622
MoBiTec
BMEG23 10 µg
434,93 €
Bacillus megaterium vector pN-StrepXa1622
MoBiTec
BMEG24 10 µg
451,41 €
Bacillus megaterium vector pSTREPHIS1525
MoBiTec
BMEG15 10 µg
291,39 €
Bacillus megaterium vector pWH1520, lyophilized DNA
MoBiTec
BMEG03 5 µg
253,13 €
Bacillus megaterium vector, pHIS1522, lyophilized DNA
MoBiTec
BMEG12 10 µg
253,13 €
Bacillus megaterium vector, pHIS1525, lyophilized DNA
MoBiTec
BMEG13 10 µg
253,13 €
Bacillus megaterium vector, pMM1522, lyophilized DNA
MoBiTec
BMEG10 10 µg
253,13 €
Bacillus megaterium vector, pMM1525, lyophilized DNA
MoBiTec
BMEG11 10 µg
253,13 €
Bacillus megaterium vector, pSTOP1622, lyophilized DNA.
MoBiTec
BMEG25 10 µg
353,38 €
Bacillus megaterium vector, pSTREP1525
MoBiTec
BMEG14 10 µg
291,39 €
Levansucrase expression positive control, HIS-Tag
MoBiTec
BMEG13C 10 µg
59,41 €
Levansucrase expression positive control, STREP-Tag
MoBiTec
BMEG14C 10 µg
59,41 €
Levansucrase expression positive control, STREPHIS-Tag
MoBiTec
BMEG15C 10 µg
59,41 €
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