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SMARTvector Lentiviral shRNA offers maximum flexibility for vector-based RNAi experiments

Because efficient gene silencing depends on both the design and level of expression of the shRNA, it is critical to choose silencing reagents where both the targeting sequence and the specific promoter driving expression are taken into consideration.

SMARTvector Lentiviral shRNAs are designed using our most advanced microRNA-based shRNA-specific rational design algorithm resulting in highly potent and specific targeting sequences. Choose from seven promoters (human and mouse CMV, human and mouse EF1 alpha, CAG, PGK, and UBC), two fluorescent reporters (TurboGFP and TurboRFP) and a no-fluorescent reporter option to tailor SMARTvector lentiviral shRNAs for your specific cells of interest and your experimental requirements. Up to ten shRNA silencing constructs are predesigned for every gene in human, mouse and rat providing broad coverage across the entire genome.

Unsure which format you need? Click here to learn about our SMARTchoice configuration platform.

SMARTvector Lentiviral shRNA

  • Target any gene in human, mouse and rat, and tailor experiments to specific cells with multiple promoter options
  • Guaranteed silencing (see below)
  • Designed using microRNA scaffold-specific attributes for highly efficient processing via the endogenous RNAi pathway
  • Available as glycerol stock and as purified, concentrated lentiviral particles
  • Standard titers of 1 x 108 TU/mL, ± 20%, and ultra-high titers of 5 x 109 TU/mL, ± 20%; functional titers determined by flow cytometric analysis of GFP-positive, transduced HEK293T cells
  • Suitable for dividing and non-dividing cell types, including difficult-to-transfect cells such as primary, neuronal and stem cells

 

Together, these attributes greatly enhance the functionality and specificity of lentiviral-mediated RNAi and reduce the toxicity associated with low titer preparations (supernatant).

Superior Dharmacon design algorithm

  • Utilization of a highly processed, patented microRNA scaffold
  • Rationally-designed, highly functional gene targeting sequences
  • Algorithm trained on more than 500 endogenous mRNA data points for functional knockdown testing
  • Incorporation of bioinformatics strategies to reduce off-target gene knockdown events
  • Protein coding genes are targeted in the ORF and 3’UTR; lncRNAs are targeted throughout the entire transcript length

 

 

 

Lentiviral particle formats

At Dharmacon, we are committed to delivering high quality lentiviral particle preparations. As such, we clone, package and concentrate each construct with strict QC controls. Due to the inherent complexity of lentiviral particle production and the quality control procedures, turnaround time is estimated to be 4 to 6 weeks. We offer the following formats:

 

Set of 3 different gene-targeting constructs

  • Ideal for evaluating multiple shRNAs targeting the same gene and ensuring mRNA knockdown in your specific cells

  • 100 µL (4 x 25 µL) or 200 µL (8 x 25 µL) total amounts at 1 x 108 TU/mL, ± 20%, functional titers determined by flow cytometric analysis of GFP-positive, transduced HEK293T cells

 

Individual gene-targeting constructs

  • Ideal for ordering one or two constructs that you have identified as being the best performers

  • 100 µL (4 x 25 µL) or 200 µL (8 x 25 µL) total amounts at 1 x 108 TU/mL, ± 20%, or 100 µL (4 x 25 µL) total amount at 5 x 109 TU/mL, ± 20%, functional titers determined by flow cytometric analysis of GFP-positive, transduced HEK293T cells

 

SMARTvector Lentiviral Positive and Negative Controls for shRNA and microRNA experiments

  • Ideal for optimizing transduction and assay conditions prior to gene-specific experimentation

  • Negative control particles allow for distinguishing sequence-specific silencing from non-specific effects

  • Positive control particles allow for confirmation of gene silencing using confirmed shRNA constructs targeting a housekeeping gene

  • 50 µL (2 x 25 µL) at 1 x 108 TU/mL, ± 20% or 100 µL (4 x 25 µL) at 5 x 109 TU/mL, ± 20%, functional titers determined by flow cytometric analysis of GFP-positive, transduced HEK293T cells

  • Click here to learn about SMARTvector controls

 

SMARTvector Lentiviral shRNA functional guarantee

When you purchase a minimum of three SMARTvector lentiviral shRNAs to the same protein-coding gene target using the optimal SMARTchoice promoter for your cell type, at least one of the shRNA constructs will reduce target mRNA levels by 70% or more when used with the vector matched non-targeting control and GAPD or PPIB positive control. Optimal promoters should be determined with the SMARTchoice promoter selection plate. mRNA levels should be measured with RT-qPCR or similar quantitative mRNA analysis no earlier than 72 hours post-transduction. The guarantee only applies to catalog numbers V3S*xxxx and does not apply to any SMARTvector shRNAs targeting lncRNA transcripts.

WIĘCEJ...

SMARTchoice Lentiviral shRNA

Sortuj wg: ▲ Nazwa produktu | Cena
Nazwa produktu / Producent Nr. katalogowy Opakowanie Cena brutto  
SMARTchoice Promoter Selection Plate
Dharmacon, Open Biosystems (część GE Healthcare)
SP-001000-01 1 each
Individual Human Lentiviral shRNA Construct
Dharmacon, Open Biosystems (część GE Healthcare)
SH-HUMAN-XX-20 200 µL 10^8 TU/mL
Individual Human Lentiviral shRNA Construct
Dharmacon, Open Biosystems (część GE Healthcare)
SH-HUMAN-XX-10 100 µL 10^8 TU/mL
Individual Mouse Lentiviral shRNA Construct
Dharmacon, Open Biosystems (część GE Healthcare)
SH-MOUSE-XX-10 100 µL 10^8 TU/mL
Individual Mouse Lentiviral shRNA Construct
Dharmacon, Open Biosystems (część GE Healthcare)
SH-MOUSE-XX-20 200 µL 10^8 TU/mL
Individual Rat Lentiviral shRNA Construct
Dharmacon, Open Biosystems (część GE Healthcare)
SH-RAT-XX-10 100 µL 10^8 TU/mL
Individual Rat Lentiviral shRNA Construct
Dharmacon, Open Biosystems (część GE Healthcare)
SH-RAT-XX-20 200 µL 10^8 TU/mL
Individual SMARTvector 2.0 CAG-TurboGFP Human Lentiviral shRNA Particles
Dharmacon, Open Biosystems (część GE Healthcare)
SH05-HUMAN-XX-10 100 µL 10^8 TU/mL
Individual SMARTvector 2.0 CAG-TurboGFP Human Lentiviral shRNA Particles
Dharmacon, Open Biosystems (część GE Healthcare)
SH05-HUMAN-XX-20 200 µL 10^8 TU/mL
Individual SMARTvector 2.0 CAG-TurboGFP Mouse Lentiviral shRNA Particles
Dharmacon, Open Biosystems (część GE Healthcare)
SH05-MOUSE-XX-10 100 µL 10^8 TU/mL
Individual SMARTvector 2.0 CAG-TurboGFP Mouse Lentiviral shRNA Particles
Dharmacon, Open Biosystems (część GE Healthcare)
SH05-MOUSE-XX-20 200 µL 10^8 TU/mL
Individual SMARTvector 2.0 CAG-TurboGFP Rat Lentiviral shRNA Particles
Dharmacon, Open Biosystems (część GE Healthcare)
SH05-RAT-XX-10 100 µL 10^8 TU/mL
Individual SMARTvector 2.0 CAG-TurboGFP Rat Lentiviral shRNA Particles
Dharmacon, Open Biosystems (część GE Healthcare)
SH05-RAT-XX-20 200 µL 10^8 TU/mL
Individual SMARTvector 2.0 CAG-TurboRFP Human Lentiviral shRNA Particles
Dharmacon, Open Biosystems (część GE Healthcare)
SH12-HUMAN-XX-10 100 µL 10^8 TU/mL
Individual SMARTvector 2.0 CAG-TurboRFP Human Lentiviral shRNA Particles
Dharmacon, Open Biosystems (część GE Healthcare)
SH12-HUMAN-XX-20 200 µL 10^8 TU/mL
Individual SMARTvector 2.0 CAG-TurboRFP Mouse Lentiviral shRNA Particles
Dharmacon, Open Biosystems (część GE Healthcare)
SH12-MOUSE-XX-10 100 µL 10^8 TU/mL
Individual SMARTvector 2.0 CAG-TurboRFP Mouse Lentiviral shRNA Particles
Dharmacon, Open Biosystems (część GE Healthcare)
SH12-MOUSE-XX-20 200 µL 10^8 TU/mL
Individual SMARTvector 2.0 CAG-TurboRFP Rat Lentiviral shRNA Particles
Dharmacon, Open Biosystems (część GE Healthcare)
SH12-RAT-XX-10 100 µL 10^8 TU/mL
Individual SMARTvector 2.0 CAG-TurboRFP Rat Lentiviral shRNA Particles
Dharmacon, Open Biosystems (część GE Healthcare)
SH12-RAT-XX-20 200 µL 10^8 TU/mL
Individual SMARTvector 2.0 hCMV-TurboRFP Human Lentiviral shRNA Particles
Dharmacon, Open Biosystems (część GE Healthcare)
SH07-HUMAN-XX-10 100 µL 10^8 TU/mL
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