Features:
Supplied in 6X Loading buffer
Description/Preparation:
The StyI digest of phage-DNA yields the 11 fragments. Lambda DNA
was completely digested by StyI, phenol extracted, ethanol
precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
The marker is ready-to-use.
Usage: 0,5 µg/lane
Concentration: 0,2 µg/µl
Number of
bands: 11 (19329*, 7743, 6223, 4254*, 3472, 2690,
1882, 1489, 925, 421, 74).
Phagen Lambda Sty I DNA
marker DNA Sizer Loading:
Agarose Gel / Polyacrylamide Gel
- Vortex gently before using
- apply 0,5 µg (agarose) or 0.5-0.9 µg (polyacrylamide gel)
per 1 mm lane
Quantification:
See the graph for the percentage of the bands per band in ng,
relating to 0.5 μg loaded marker. Use the same volume of DNA and
marker. Additionally the concentration of loading buffer in
samples and marker should be equal.
Note:
Dilute in TE or other buffer of minimal ionic strength. DNA may
denature if diluted in dH2O and subsequently heated.
*The cohesive ends (12b cos site of bacteriophage) of
fragments 19329 bp and 4254 bp may anneal to and form the
additional band. These fragments may be separated by heating to
65oC for 5 min and then cooling on ice for 3 min.
Transportation: Shipped
on blue ice or room temperature
Storage: at
-20°C for 24 months
