1000bp/1kb DNA ladder (no loading dye)
Opis
1000 bp/1 kb DNA Marker from GeneON is composed of 13 individual DNA fragments which are re-dissolved in storage buffer.
Description
The 1000 bp/1 kb DNA Ladder has a number of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 13 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The 1000 bp/1 kb DNA Ladder was designed for precise qualitative and approximately quantification of DNA mass. There are no unspecific bands besides the fragments. The 500 bp, 1000 bp and 3000 bp fragment are used as reference band.
Usage: 1 µg/lane
Concentration: 0,2 µg/µl
Storage Buffer: 10 mM Tris-HCl (pH 8.0), 1.0 mM EDTA, 50mM NaCl
Tracking Dye: 6X DNA Loading dye required
Number of bands: 13 fragments
Loading: 0.5 - max. 1.2 µg/lane in 1.7 - max. 2.0 % Agarose. We recommend to add the loading dye in amount 1/10 from the DNA Ladder volume
Transportation: shipped on blue ice or room temperature
Storage: at -20°C for 24 months
Number of bands and approx. DNA Mass:
base pairs 250 500 750 1000 1500 2000 2500 3000 4000 5000 6000 8000 10000
DNA mass ng 20 30 60 210 50 60 70 200 60 60 60 60 60
Dane techniczne
Opakowanie | 50 µg |