Produkty
Producenci
100bp plus DNA Ladder (no loading dye)
Opis
The 100 bp PLUS DNA Ladder has a number of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The 100 bp PLUS DNA Ladder was designed for precise qualitative and approximately quantification of DNA mass. There are no unspecific bands besides the fragments. The 500bp fragment is used a reference band.
Usage: 1 µg/lane
Concentration: 0,2 µg/µl
Storage Buffer: 10 mM Tris-HCl (pH 8.0), 1.0 mM EDTA, 50mM NaCl
Tracking Dye: 6X DNA Loading dye required
Number of bands: 11 fragments
Number of bands and approx. DNA Mass:
| Base pairs | 100 | 200 | 300 | 400 | 500 | 600 | 700 | 800 | 900 | 1000 | 1500 |
| DNA mass ng | 20 | 20 | 30 | 50 | 150 | 80 | 70 | 110 | 120 | 200 | 150 |
Loading: 0.5 - max. 1.2 µg/ lane in 1.7 - 2.5 % Agarose. We recommend to add the loading dye in amount 1/10 from the DNA Ladder volume
Storage: at -20°C for 24 months
Dane techniczne
| Opakowanie | 50 µg |