5-Fusion Multiplex (Negative Control)
Opis
Format: FFPE
The 5-Fusion Multiplex (Negative Control) FFPE RNA Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of targeted NGS, RT-PCR and RT-qPCR assays aimed at detecting gene fusions. Each FFPE section is composed of a formalin-fixed, paraffin-embedded (FFPE) cell line verified not to contain EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 fusions.
With this product you are able to:
• Evaluate your workflow integrity from pre-analytical RNA extraction through to fusion detection
• Negative control for EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 fusion detection panels
Targeted NGS, endpoint RT-PCR and RT-qPCR platforms give researchers much greater power when profiling tumor samples. However, they present challenges surrounding sample variability, RNA extraction procedures, RNA quantification, platform bias, and potential failure to detect biomarkers.
Technical Data
Genes Covered: Negative for EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3
Cosmic ID: COSF463 (EML4-ALK); COSF1272 (CCDC6-RET); COSF1197 (SLC34A2-ROS1); COSF1330 (TPM3-NTRK1); COSF572 (ETV6-NTRK3)
Fixation Method: 10% Formalin
Section Size: 10µm
Product Information
Intended Use: For routine performance monitoring (Research Use Only)
Unit Size: 1 FFPE Section
General Information
Storage: 4°C
Expiry: 12 months from the date of manufacture
Quality Control
Extractable RNA / Expected Yield: ≥ 100 ng RNA per section using the Maxwell 16 LEV RNA FFPE Purification Kit from Promega
Biomarker-specific mRNA amplifiability: Absence of EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 RNA fusion transcripts confirmed by endpoint RT-PCR
TATA-binding protein (TBP) mRNA amplifiability control: RNA expression confirmed by endpoint RT-PCR
Quantification: Qubit RNA HS kit from ThermoFisher as per the manufacturer’s instructions