ACRYLAMIDE

Molecular Biology Reagent, ≥99%, White Crystalline Powder

Solubility: Soluble in water (to at least 40% w/v, but has been reported to be soluble in water up to 215 g/100 ml at 30°C.1), methanol (155 g/100 ml), ethanol (86 g/100 ml), alcohol, acetone (63.1 g/100 ml); only minimally soluble in benzene or heptane.1 Solutions should be kept at +4°C in the dark and can be kept for up to one year.

For a 30% stock solution dissolve 29 grams acrylamide and 1 g of N,N'-methylene-bis- acrylamide in a total volume of 60 ml distilled deionized water. Heat the solution slightly (approximately 37°C) and stir until the acrylamide and bisacrylamide are dissolved. Bring the volume to 100 ml with distilled, deionized water and stir. Sterilize the solution by filtration through a Nalgene filter (0.45 micron pore size). Check the pH of the solution and make sure it is at 7.0 or less.

For a 40% stock solution dissolve 380 grams acrylamide and 20 g of N,N'-methylene-bis- acrylamide in a total volume of 600 ml distilled deionized water. Heat the solution slightly (approximately 37°C) and stir until the acrylamide and bisacrylamide are dissolved. Bring the volume to 1000 ml with distilled, deionized water and stir. Sterilize the solution by filtration through a Nalgene filter (0.45 micron pore size). Check the pH of the solution and make sure it is at 7.0 or less.