Produkty
Producenci
APROTININ Analytical Grade
Opis
Biochemical Physiological Actions
Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.
| Source | Bovine Lung |
| Specific Activity | ~6000 KIU/mg |
| Typical Working Concentration | Equimolar with protease (1-2 ug/mL). |
| Unit Definition | KIU: One KIU (Kallikrein Inactivating Unit) is identical to the quantity of protease inhibitor that has the ability to inhibit 2 Kallikrein units by 50% under optimal conditions. |
Application Notes
Small amounts of aprotinin can be added to tubes of drawn blood to enable laboratory measurement of rapidly degraded proteins such as glucagon. Aprotinin can be labelled with fluorescein isothiocyanate. The conjugate retains its antiproteolytic and carbohydrate-binding properties and has been used as a fluorescent histochemical reagent for staining glycoconjugates (mucosubstances) that are rich in uronic or sialic acids. In cell biology aprotinin is used as an enzyme inhibitor to prevent protein degradation during lysis or homogenization of cells and tissues .
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Dane techniczne
| Opakowanie | 10 mg |