Produkty
Producenci

Accell Set of 4 Upgrade

nr kat.: EU-RAT-XX-0020
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Producent: Horizon Discovery Group
nr kat.: EU-RAT-XX-0020

Opis

Accell siRNA achieves what no other RNAi product can claim: delivery into difficult-to-transfect cells without transfection reagents, virus, or instrumentation. Researchers around the world are achieving targeted gene silencing in neuronal, immune, and primary cells that had previously been beyond the reach of conventional RNAi products due to toxicity caused by transfection reagents or undesirable viral responses.
Accell siRNA is available as pre-designed, genome-wide SMARTpool and single siRNA reagents. Simply search for your gene of interest, and choose from the available product formats and quantities.
 
Highlights
  • Accell siRNA enters cells without the need for transfection reagents, virus (or viral vectors), or instruments
  • Novel siRNA modifications facilitate uptake, stability, specificity, and knockdown efficiency
  • Proven performance in neuronal, immunological, primary, and other difficult-to-transfect cell types
  • Appropriate for use in vivo, due to stabilizing modifications
  • Extended-duration knockdown with optimized continuous application
 
Product Formats
 
SMARTpool: A mixture of 4 siRNA provided as a single reagent; providing advantages in both potency and specificity.
Set of 4: A convenient option for purchasing aliquots of all 4 individual siRNAs targeting a single gene.
Set of 4 Upgrade: Discounted price on the Set of 4 based on a concurrent or prior purchase of the SMARTpool reagent for the same gene.
Individual siRNAs: Select 1, 2, 3 or 4 individual siRNAs per gene.
 
Start your gene search   
 
 
Peer-reviewed publications demonstrate effective silencing in numerous cell types
 
Human Suspension Cells: BDCM, IM-9, Jurkat, K662, THP-1, Jeko-1, and MCL
Human Adherent Cell lines: A-375, ARPE-19, CaCo-2, CG4, DLD-1, DU 145, EC52, GTM-3, MR32, LAN5, LNCaP, MIA, PACa-2, NCl/ADR-RES, OVCAR-3, OVCA-420, PGA1, PNT2, SH-SY5Y, SK-N-BE and SK-N-SH neuroblastoma, SK-BR3, SKOV3, TE15, U251, U87MG, U937
Mouse and rat Cell Lines: ES-D3, H9c2, LβT2, LNK, mouse oviduct epithelial cells, mProx24, PC-12
Human Differentiated Stem Cells: Adipocytes and osteoblasts from hMSC
Human Primary Cells: HUVEC, HUASMC, hMSC, PBMC, astrocytes, hypothalamic neurons, T cells, NK cells, macrophages, immortalized B cells, esthesioneuroblastoma cells, hepatocytes, keartinocytes, bronchial smooth muscle cells
Mouse and Rat Primery Cells: Cortical neurons, hippocampal neurons, striatial neurons, Nalve CD4+ T cells, LN T-cells, D1 T cells, RAW264.7, macrophages, hepatocytes, neonatal vertricular cardiomyocytes, fibroblasts derived from genetically modified mice, bone-marrow-derived DC, NOD CD4+CD25-splenic cells, NOD BMMs, VSMCs, somatolactotroph GH3, McArdle 7777 cells, (hepatoma cell line), osteclast precursors, calvarial (cranial) organ, pituitary cell typesderived from primary tumors
 
Ordering guidelines
 
Due to the unique nature of Accell siRNA delivery, it requires a higher working concentration than conventional siRNAs. This table provides the approximate number of reactions (wells) at recommended 1μM Accell siRNA working concentration in different plate formats (assuming no loss from pipetting).

Manuals
 
Product Insert           Delivery Protocol
 
References
 
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