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AminoLink™ Plus Immobilization Kit, 2 mL

nr kat.: 44894
Opakowanie: 5 x columns
Cena brutto: do wyceny
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Opis

The AminoLink Plus Immobilization Kit contains AminoLink Plus Coupling Resin and additional reagents needed to perform 5 protein immobilizations, with expected yields of 2 to 40 mg protein per column. The AminoLink Plus Coupling Resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Features of AminoLink Plus Coupling Resin:

• AminoLink Plus CouplingResin—aldehyde-activated crosslinked 4% beaded agarose
• Ideal for antibodies and other proteins— immobilize molecules via primary amines (-NH2)
• Flexible coupling conditions—efficient (>85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent); regular (PBS, pH 7.2) and enhanced (citrate-carbonate, pH 10) coupling protocols provided
• Stable, permanent immobilization—Coupling reaction esults in stable, leak-resistant secondary amine bond between resin and ligand
• Better than immobilization to CNBr-activated agarose—bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
• Versatile and reusable—prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions

AminoLink Plus Resin is beaded agarose support that has been modified to contain aldehyde groups. Antibodies, other proteins, or any molecules with primary amines can be conjugated to the resin by an optimized form of reductive amination. Once an antibody or other ligand is immobilized, the prepared affinity resin can be used for a variety of affinity purification methods involving batch or column chromatography. The resin and linkage are stable in binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for at least 10 rounds of affinity purification.

Contents & storage
Sufficient For: Immobilizing 2 to 40 mg of protein per column
• AminoLink Plus Columns, 2 mL, 5 columns
• PBS Pack (makes 500mL), 1 pack
• Citrate-Carbonate Buffer Pack (makes 500mL), 1 pack
• Sodium Cyanoborohydride Solution, 5M, 0.5 mL
• Quenching Buffer, 60 mL
• Wash Solution, 240 mL
• Column Accessories

Dane techniczne

Opakowanie 5 x columns

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