Produkty
Producenci

Bacillus megaterium protoplast, strain WH320

nr kat.: BMEG02
Opakowanie: 5x500 µl
Cena brutto: do wyceny
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Opis

    hp-Vector Expression Systems for Bacillus megaterium


  • High performance vectors with optimized sequences
  • Protein yield up to 10 times enhanced compared to protein expression with basic plasmid
  • Plasmids with established MCS
  • Encoding C- or N-terminal His-tag for versatile purification (native, 6xHis-tag)
  • Secretion with LipA or YocH signal peptide up to 9-fold increased
  • Vector with two ribosome binding sites (2RBS) for simultaneous dual expression available

 

 



Relative fluorescence of an optimized hp-Vector (red; square) and a basic plasmid (blue; circle) over time [h].

MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable to most applications and protein purification methods. These include vectors carrying two different signal peptide sequences, a 6xHis-tag, a Strep-tag, or two ribosome binding sites (2RBS) for simultaneous dual expression.

With our new hp-vectors yields up to 10-fold higher compared to the basic plasmids are possible. All plasmids have established multiple cloning sites (MCS) for versatile cloning. Furthermore, we offer vectors encoding C- or N-terminal His-tags for easy purification. The protein secretion with LipA or YocH signal peptides is up to 9-fold increased. Induction of protein expression of all vectors is achieved by the tightly regulated and efficiently inducible xylose operon.

 

Versatile system with a wide range of vectors

The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small- and large-scale. B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by external alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.

 

 

 

 

Soluble protein fractions 7.5 h after induction of heterologous gene expression.

Dane techniczne

Opakowanie 5x500 µl

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