Bambanker™ Direct - freezing medium

BAMBANKER™ DIRECT is a novel cell freezing medium, which can be added directly to culture medium without centrifuging the collected cells. Then, after suspending the cells the user can freeze and preserve the cells in a deep freezer at -80°C.
This medium is especially interesting for all High ThroughPut cell culture users as well as for everybody working with hybridoma cells (suspension cells). This medium is pretty much unique.

Features

• Tiring preparation steps for freezing are not necessary
• No progrmmable freezer is required
• Add Bambanker™ DIRECT to culture medium without centrifuging the cells
• Ready-to-use without dilution
• Rapid and long-term freezing and preservation in a deep freezer (-80 °C)
• Serum-free

Cell lines succesfully stabilized by BambankerTM Direct:

• C2C12 (mouse skeletal muscle cell line)
• Daudi (human B cell line)
• HEK293; HEK293T (human embryonic kidney cell line)
• HeLa/HeLa S3 (human cervical cancer cell line)
• HepG2 (human liver cancer cell line)
• Jurkat (human leukemia T cell line)
• K562 (human chronic myelogenous leukemia cell line)
• KATO (human gastric epithelial cell line)
• MDCK (canine kidney tubular epithelial cell line)
• NIH3T3 (mouse embryonic skin cell line)
• OKT4 (mouse hybridoma)
• OP9 (mouse myeloid stromal cells)
• PC12 (rat adrenal medulla cell line)
• SNL (mouse embryonic fibroblasts)
• ES (mouse embryonic stem cells)
• MEF (mouse embryonic fibroblasts)
• Vero (African green monkey kidney cell line)
• OP9 (mouse myeloma stroma cells)
• P3U1 (mouse myeloma cell line)

Fig.1 Three different cell lines before freezing and 12 months after. Cells were stored at -80°C.

Operating Procedure:

 Freezing of suspended cells
1.) Take out cells in a culture process from an incubator.
2.) Measure the same amount of Bambanker™ Direct as that of the culture medium. Add this
     amount to the cell culture.
3.) Conduct a gentle pipetting so that the culture medium and Bambanker™ Direct are uniformly
     mixed.
4.) Transfer the mixed solution to cryo-tubes for freezing and preservation.
5.) Freeze cells at -80 °C.
 

Freezing of adherent cells
1.) Take out cells in a culture process from an incubator.
2.) Remove the culture medium.
3.) Rins the cells twice with PBS. Then add cell detachement solution and heat it up.
4.) Add the same amount of Bambanker™ Direct as that of the detachement solution.
5.) Conduct a gentle pipetting so that the detachement solution and Bambanker™ Direct are
      uniformly mixed.
6.) Transfer the mixed solution to cryo-tubes for freezing and preservation.
7.) Freeze cells at - 80 °C.