Bio-Star Mastermix with SYBR (2X) Low-ROX

Features:
- Enzyme with hot start capability increases reaction specificity and sensitivity, optimized
- DFS-Taq PLUS DNA polymerase activation requires not more than 5 min heating
- High selectivity and reaction yield
- Reduced preparation time

Applications:
- Real-time PCR with intercalating dye SYBR Green I and ROX as reference dye
- Conventional PCR
- High-throughput PCR
- Genotyping
- For detection of bacterial DNA

Platforms:
(ABI) 7500, 7500 Fast, ViiA 7, QuantStudio 12K; Stratagene Mx4000, Mx3005P, and Mx3000P

Description
Bio-Star qPCR-Mastermix SYBR Blue (2×) is developed for quantitative real-time PCR with fluorescent dye SYBR Green I. The Mastermix contains all components, except template and primers, for successful PCR.
- The mix is optimized for efficient and reproducible hot-start real-time PCR of genomic, plasmid and viral DNA samples.
- The solution contains substances that increase half-life and processivity of DFS-Taq PLUS DNA polymerase by enhancing its stability during PCR.
- It includes components that influence primer annealing temperature and characteristics of template melting thus enabling to increase the specificity of PCR and use templates with complicated structure.
- DFS-Taq Plus DNA polymerase is inactive at room temperature because of monoclonal antibodies.
- The inert dye allows control when using multi-well plates. Use of the kit saves time and minimizes contamination risk due to reduced number of pipetting steps.

Components and Mixture
2X Bio-Star qPCR-Mastermix SYBR Blue contains:
- 100 mM Tris-НCl (рН 8.5 at 25 °С), 100 mM KCl, 0.4 mM each of dNTP, 3 mM MgCl2, 0.06 U/µl DFS-Taq DNA Polymerase, 0.025% Tween 20, stabilizers and enhancers, SYBR Green I, SYBR Green I, 60 nM ROX fluorescent dye,
- Water Mol.Bio Grade 2 x 1,25 ml

Storage and transportation: at -20 °С; not more than 50 thawing-freezing cycles. shipping with blue ice or at room temperature

Storage terms: up to 24 mounts
 
Stability tests / Quality control / Comparison

• Exodeoxyribonuclease activity: DNA was stable after incubation of 1 µg fragment of phage lambda DNA in the presence of 25 µl of Bio-Star qPCR Mastermix (2×) in 50 µl reaction solution at 37 °C and 70 °C for 4 h.
• Test for bacterial DNA contamination
• Test for Hot -Start ( we test activity with or without MAB at various temperatures
• Exodeoxyribonuclease activity
• DNA was stable after incubation of 1 μg fragment of phage lambda DNA in the presence of 25 μl of BioMaster HS-qPCR (2×) in 50 μl reaction solution at 37 °C and 70 °C for 4 h.
• Ribonuclease activity
• Absence of ribonuclease activity was confirmed after incubation of 1 μg of 5’-[P32]-labeled RNA fragment in the presence of 25 μl of BioMaster HS-qPCR (2×) in 50 μl reaction solution at 37 °C for 4 h.

Stability:
One month at room temperature does not reduce PCR efficiency