Produkty
Producenci
Blue 1000bp/1kb DNA ladder (ready-to-use)
Opis
1kb ladder BLUE from GeneON is composed of 13 individual DNA fragments which are re-dissolved in storage buffer.
Description/Preparation: The 1 kb ladder BLUE is a pre-mixed, ready-to-load molecular weight marker containing a dye which serves as visual aids to monitor the progress of migration during agarose gel electrophoresis. The DNA marker consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 13 bands suitable for use as molecular weight standards for agarose gel electrophoresis that range in size from 250 to 10.000 base pairs. The 1.000 bp and 3.000 bp fragments have increased intensity relative to the other bands on ethidium bromide-stained agarose gels and serve as reference indicators. All fragments are blunt-ended.
Usage: max. 1 µg/lane
Concentration: 0,1 µg/µl
Storage Buffer/Tracking Dye: 10 mM Tris-HCl (pH 8.0), 1.0 mM EDTA, 50mM NaCl, optimized blue loading dye
Gel-picture: 1 μg of 1000 bp/1 kb Ladder visualized by ethidium bromide staining on 1,0 % TAE agarose gel
Number of bands: 13 fragments
Number of bands and approx. DNA Mass:
base pairs 250 500 750 1000 1500 2000 2500 3000 4000 5000 6000 8000 10000
DNA mass ng 20 30 60 210 50 60 70 200 60 60 60 60 60
Loading: 0.25 - max. 1.0 µg/ lane in 1.7 - max. 2.5 % Agarose.
Transportation: shipped on blue ice or room temperature
Storage: at -20°C for 24 months
Dane techniczne
| Opakowanie | 50 µg |