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BlueEasy Prestained Protein Marker (500 µl)

BlueEasy Prestained Protein Marker (500 µl)
Cena brutto: 494,21 zł
Cena netto: 401,80 zł
szt.
Opakowanie:
500 µl
Kod produktu:

MWP06

Producent:

The BlueEasy Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

  • Broad range: 6.5 - 270 kDa.
  • High performance: 10 bands with 3 colours (blue, orange and green)
  • Ready to use: Supplied in a loading buffer for direct loading on gels.
  • Easy to identify: The 52 (green), 30 kDa and 270 kDa (orange) are reference bands.
  • Sharp bands

 

Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (62,5 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 1 mM EDTA, and 33 % (v/v) Glycerol).

Quality Control
Under suggested conditions, BlueEasy Prestained Protein Ladder resolves 10 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose and other membranes.

Storage
Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

Loading
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
1.5~2.5 μl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.

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