BlueStar PLUS Prestained Protein Ladder (500 µl)
Opis
Broad range: 9 - 245 kDa.
- High performance: 12 bands with 3 colours (blue, red and green)
- Ready to use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: The 25 (green) and 75 kDa (red) are reference bands.
- Sharp bands
Applications
The BlueStar PLUS Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Quality Control
Under suggested conditions, BlueStar PLUS Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane.
Storage
Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C.
Loading
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
1.5~2.5 μl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.
Dane techniczne
Opakowanie | 500 µl |