BlueStar Prestained Protein Ladder (500 µl)
Opis
- Broad range: 10 - 180 kDa.
- Ready to use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes the 28 and 75 kDa reference bands coupled with an green dye and an orange dye, respectively.
- Sharp bands
The BlueStar Prestained Protein Ladder is a three color protein standard with 10 prestained proteins covering a wide range molecular weights from 10 to 180 kDa.
The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.
The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
Applications
- Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.
- Monitoring of protein transfer onto membranes during Western blotting.
- Sizing of proteins on SDS-polyacrylamide gels and Western blots.
Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.
Storage Buffer
20 mM Disodium hydrogen phosphate pH 8.0 (at 25°C ), 2 % SDS, 0.2% Triton X-100,10 mM 2-Mercaptoethanol, 6 M Urea and 27% (v/v) Glycerol.
Storage
Store at -20°C for 12 months.
Stable at +4°C for 3 months.
Recommendations for Loading
1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly, to ensure that the solution is homogeneous.
3. Load the following volumes of the ladder on SDS-polyacrylamide gel:
- 5 μl per well for mini-gels, 3 μl per well for blots;
- 10 μl per well for large gels, 6 μl per well for blots
Dane techniczne
Opakowanie | 500 µl |