Produkty
Producenci
DNA Polymerase I (E. coli)
Opis
escription: DNA Polymerase I (E. coli) is a non-thermostable DNA polymerase with inherent 3´→ 5´ (proofreading) and 5´→ 3´ exonuclease activities, in addition to a lower and non-specific ribonuclease H activity. The 5´→ 3´ exonuclease activity removes nucleotides ahead of the growing DNA chain, allowing nick-translation. Thus, DNA Polymerase I (E. coli) displays no strand-displacement activity and may be used for DNA labelling by nick-translation, in conjunction with DNase I, or second-strand cDNA synthesis, in conjunction with RNase H. DNA Polymerase I (E. coli) accepts modified nucleotides (e.g. biotin-, digoxigenin-, fluorescent-labelled nucleotides) as substrates for the DNA synthesis.
Features:
– 3´→ 5´ proofreading activity
– 3´→ 5´ and 5´→ 3´ exonuclease activities
– No strand-displacement activity
– Non-thermostable DNA polymerase: is heat inactivated at 75 °C for 20 min
Applications:
– DNA labelling by nick-translation
– Second-strand synthesis of cDNA
Specifications:
| Enzyme concentration: | 10 U/μL |
| Source: | Recombinant E. coli strain |
| Optimal reaction temperature: | 15-37 °C |
| Heat inactivation: | 75 °C for 20 min |
| Storage conditions: | Store at -20 °C |
| Shipping conditions: | Shipped at dry ice |
Components:
– DNA Polymerase I (E.coli) (10 U/μL)
– Reaction Buffer (10x)
Dane techniczne
| Opakowanie | 500 U |