Deoxyribonuclease I (RNase free), supplied with MnCl2
Opis
Concentration: 1u/µl
- In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion.
- In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides.
- Recombinant enzyme
- Purified from non-animal host with a lower level of intrinsic RNases
- Preparation of DNA-free RNA
- Removal of template DNA following in vitro transcription
- Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
- DNA labeling by nick-translation in conjunction with DNA Polymerase I
- Studies of DNA-protein interactions by DNase I, RNase-free footprinting
- Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used
Dane techniczne
Opakowanie | 1000 U |