Produkty
Producenci

Deoxyribonuclease I (RNase free), supplied with MnCl2

nr kat.: EN0525
Opakowanie: 1000 U
Cena brutto: do wyceny
ilość szt.

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Opis

Concentration: 1u/µl

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.
The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions.
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  • In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion.
  • In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides.
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Highlights
  • Recombinant enzyme
  • Purified from non-animal host with a lower level of intrinsic RNases
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Applications
  • Preparation of DNA-free RNA
  • Removal of template DNA following in vitro transcription
  • Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
  • DNA labeling by nick-translation in conjunction with DNA Polymerase I
  • Studies of DNA-protein interactions by DNase I, RNase-free footprinting
  • Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used
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Note
DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.

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Opakowanie 1000 U
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