DharmaFECT kb DNA transfection reagent - for plasmid transfection

A unique transfection reagent from our DharmaFECT product line for introduction of DNA into cultured cells with high efficiency and low cytotoxicity

DharmaFECT transfection reagents have proven to be extremely valuable tools for introducing small RNAs into cultured cells.  DharmaFECT kb has the same reliable efficiency and low of toxicity as our RNA-specific reagents, but is specifically engineered for delivery of plasmids to your cells of interest.

Highlights

• High transfection efficiency of plasmid DNA into a variety of cell types, including: HeLa, HEK293T, U2OS, and MCF-7
• Large dynamic range with low toxicity
• Works well for co-transfection of Cas9 nuclease and sgRNA plasmids
• Simple and familiar protocol
• Works equally well in the presence or absence of serum

Successful CRISPR-Cas9 gene editing utilizing co-delivery of Edit-R Lentiviral sgRNA and Cas9 expression plasmid using DharmaFECT kb transfection reagent


U2OS cells were transfected with equal amounts of Edit-R hCMV-mKate2-Cas9 Expression plasmid (Cat #U-004100-120) and Edit-R Human PPIB Lentiviral sgRNA plasmid in a 96-well format. Transient transfections were done with increasing amounts of total DNA (50 to 200 ng) and DharmaFECT kb transfection reagent (Cat #T-2006-01), 0.2 to 0.8 µL per well. The percentage of gene editing was estimated 72 hours after transfection by DNA mismatch detection assay using T7EI with gel densitometry.


TurboGFP expression from SMARTvector lentiviral shRNA plasmid as an indicator of successful delivery with DharmaFECT kb transfection reagent


HEK293T cells were transfected with 1 µg SMARTvector Human AKT1 Lentiviral shRNA plasmid (Cat #V3SH11240-224972267) in a 24-well format. Transient transfections were done with DharmaFECT kb transfection reagent (Cat #T-2006-01) at 3 µL per well. Images were taken 48 hours after transfection.