DyNAmo™ HS SYBR® Green qPCR Kit (with ROX)

The master mix in Thermo Scientific DyNAmo HS SYBR Green qPCR Kit contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR. Only template DNA and PCR primers need to be added by the user. The modified DNA polymerase in this kit incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex.

ROX passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization.

Highlights

• Strong specificity: DyNAmo HS SYBR Green master mix minimizes the formation of unwanted primer-dimers and extension of non-specifically bound primers during reaction setup.
• Highly sensitive detection: Optimized master mix allows reproducible detection of low-copy number templates.
• Broad dynamic range: DyNAmo HS SYBR Green qPCR Kit detects as few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude.
• Convenient to use: 2X master mix, one tube only. ROX passive reference dye provided in a separate vial.

Includes

• 2X Master Mix (containing a hot start version of a modified Thermus brockianus DNA polymerase, SYBR Green I dye, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP)
• 50X ROX passive reference dye

Manual