EGFR Gene-Specific Multiplex Reference Standard (FFPE) 5% AF

Format: FFPE

The Gene-Specific FFPE Reference Standards are a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS assays that detect somatic mutations. These standards are available specifically in either an EGFR Multiplex (1 and 5% allelic frequency range) or a KRAS Multiplex (5% allelic frequency range).

With this product you are able to:

• Evaluate of workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics
• Analyze the sensitivity of your workflow
• Optimize and validate new cancer panels and routinely monitor the performance of your assay

Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms.

The Gene-Specific Multiplex Reference Standard portfolio covers multiple endogenous SNPs, insertions and deletions at defined allelic frequency ranges. The Gene-Specific Reference Standards cover EGFR or KRAS-specific mutations. These standards may be diluted with a matched wild type while the background mutations remain consistent. The Gene Specific Multiplex Standards are available in genomic DNA and FFPE format, which allows you to validate both the pre-analytical and analytical portion of your workflow. The FFPE format allows you to evaluate and optimize DNA extraction protocols without wasting precious patient samples.

By multiplexing multiple gene-specific mutations in a single standard, the Gene-Specific Multiplex allows researchers to maximize the information obtained from their assay, and confirm that they are able to detect a range of potential mutants in a single gene.

Technical Data

Genes Covered: EGFR

Cosmics: 950+

Fixation Method: 4% Formalin

Section Size: 15µm or 20µm

Cell Density: 3 x 10⁸ cells per block. Approx. 3.5 x 10⁵ cells per section

Expected DNA Yield: ≥ 400 ng using Promega Maxwell LEV Plus Extraction kit

 


Unit Size: 1 FFPE Section

Extractable DNA: ≥ 400 ng per section using Promega Maxwell LEV Plus FFPE DNA Extraction kit

Intended Use: For routine performance monitoring (Research Use Only)

General Information

Allelic Frequency: 5%

Storage: 4˚C

Expiry: 48 months from the date of manufacture

Cell Line Background: RKO

Quality Control
Genotype: Sanger sequencing of locus specific PCR
Quality: Agarose gel electrophoresis
Quantification: Quantifluor™