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EXO I - SAP clean up kit

Cena brutto: 773,82 zł
Cena netto: 716,50 zł
szt.
Opakowanie:
500 µl
Kod produktu:

S610

Producent:

The Kit contains two hydrolytic enzymes, recombinant Shrimp Alkaline Phosphatase (rSAP) and Exonuclease I (Exo I).

The combination of these enzymes ensures complete dephosphorylation of dNTPs and degradation of residual primers, enabling downstream applications, such as sequencing, genotyping, cloning or SNP analysis.

The reagents are active in commonly used PCR buffers and eliminates the need for additional buffer exchange.

Content:  SAP-Exo Mix (red cap)

Notes: avoid multiple freeze / thaw cycles

 
Applications:

SAP-Exo Kit is a quick, easy and reliable enzymatic cleanup reagent for PCR product clean up.

• Removes excess primers and dNTPs

• Fast 20 minute protocol

• Add directly to PCR product

• 100% Sample Recovery

• Scalable for different reaction sizes

• No interference on downstream applications

• Easy to automate

 

Protocol:

-Add 2µl of SAP-Exo mix directly to 5µl PCR product (or scale up, i.g. 4µl SAP-Exo mix to 10µl PCR product) and mix well

-Incubate for 10 min at 37°C

-Inactivate for 10 min at 80°C

 

PCR products are ready for downstream applications without any need for further processing.

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