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Enzymatic Shearing Kit

nr kat.: 500165
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Cena brutto: do wyceny
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Opis

Chromatrap is a more efficient, sensitive and robust method of ChIP

Compared to standard methods, Chromatrap® is:

  • FASTER
  • EASIER to use
  • MORE SENSITIVE especially for low-abundant targets.

 


What is Chromatrap?

Chromatrap® technology
Chromatrap® kits use spin columns or microplates which contain discs of a revolutionary inert, porous polymer to which protein A or G has been covalently attached. This patented format is unique. During an assay, the chromatin/antibody complex is selectively retained by the disc. Washing with three buffers and an elution step are all that is required to obtain the selectively enriched DNA, making Chromatrap® more efficient in the laboratory.
 

 

What does this mean in the laboratory?

Chromatrap® outperforms other chromatin immunoprecipitation assays because it has:

  • Robust signal to noise, typically 2-3 times better than standard methods
  • Low non-specific binding of the inert Chromatrap® discs
  • No DNA clean-up required for qPCR
  • High surface area and molecular mixing ideal for low abundant targets and challenging cell types.
  • Wide dynamic range make is suitable for ChIPseq
  • 96 well format for high throughput analysis
  • Allows more ChIP assays to be performed from a single sample

 

High surface area and flow-through characteristics are key to efficiency.

 

 

Solid phase scaffold

  • Inert material
  • Open structure
  • Pro A/G bound to surface


Flow through process

  • Promotes molecular movement
  • Better sample mixing
  • Minimises non-specific binding

 


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