Enzymatic Shearing Kit
Opis
Chromatrap is a more efficient, sensitive and robust method of ChIP
Compared to standard methods, Chromatrap® is:
- FASTER
- EASIER to use
- MORE SENSITIVE especially for low-abundant targets.
What is Chromatrap?
Chromatrap® technology
Chromatrap® kits use spin columns or microplates which contain discs of a revolutionary inert, porous polymer to which protein A or G has been covalently attached. This patented format is unique. During an assay, the chromatin/antibody complex is selectively retained by the disc. Washing with three buffers and an elution step are all that is required to obtain the selectively enriched DNA, making Chromatrap® more efficient in the laboratory.
What does this mean in the laboratory?
Chromatrap® outperforms other chromatin immunoprecipitation assays because it has:
- Robust signal to noise, typically 2-3 times better than standard methods
- Low non-specific binding of the inert Chromatrap® discs
- No DNA clean-up required for qPCR
- High surface area and molecular mixing ideal for low abundant targets and challenging cell types.
- Wide dynamic range make is suitable for ChIPseq
- 96 well format for high throughput analysis
- Allows more ChIP assays to be performed from a single sample
High surface area and flow-through characteristics are key to efficiency.
Solid phase scaffold
- Inert material
- Open structure
- Pro A/G bound to surface
Flow through process
- Promotes molecular movement
- Better sample mixing
- Minimises non-specific binding
Video protocol Presentation Brochure Protocols
Available options
Dane techniczne
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