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EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII)

nr kat.: K1441
Opakowanie: 200 react.
Cena brutto: do wyceny
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Opis

Features

  • Fast – complete digestion of genomic DNA in 1 hour at 37°C.
  • Efficient – specially formulated enzymes for epigenetic studies of DNA methylation status.

 

Applications
CpG methylation analysis at 5'-CCGG-3' loci.

Description

5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression.
The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyze DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5'-CCGG-3' tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected.
The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.

Quality Control

Functional Testing
The kit is functionally tested in Epi MspI and Epi HpaII digestion of human blood genomic DNA, spiked with methylated and unmethylated pUC19DNA/SmaI. Unmethylated plasmid and genomic DNA are completely cleaved in one hour with both enzymes. Methylated plasmid and genomic DNA are not cleaved by Epi HpaII, but cleaved by Epi MspI.

Components

Epi Mspl 200 µl
Epi HpaII 200 µl
10X EpiBuffer 1 ml
Control pUC19/SmaI DNA Unmethylated (0.5 µg/µl) 20 µl
Control pUC19/SmaI DNA CpG Methylated (0.5 µg/µl) 20 µl

 

 

Dane techniczne

Opakowanie 200 react.

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