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Producenci

FastGene ScaI

nr kat.: FG-ScaI
Opakowanie: 250 react.
Cena brutto: 267,28 zł 267.28
Cena netto: 217,30 zł
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Producent: Nippon
nr kat.: FG-ScaI

Opis

  • No Star Activity
  • Highest Purity
  • Restriction in just 5 minutes with the FastCut buffer
  • Perfect use for Cloning experiments

 

Overhang CpG Methylation
Blunt Not sensitive
Incubation Temp. Heat Inactivation
37°C 80°C for 20 min

Activity in FastGene Buffer

Buffer I Buffer II Buffer III Buffer IV FastCut Buffer
Not recommended Not recommended 100% Not recommended 100%
Supplied Buffer
Enzyme Type
Buffer III Type IIP
Isoschizomeres
ZrmI
Recognition Sequence
Amount
AGT/ACT
TCA/TGA
1000 units, 4 units/µl

 

ENZYME FINDER


Different Types of restriction enzymes

Based on the structure, cofactor requirements and specificity of cleavage there are four types of restriction enzymes (Types I, II, III, and IV).

Type I restriction enzymes cleaves the DNA at a random location far away from the recognition sequence. These enzymes require both ATP and S-adenosyl-L-methionine to function.

Type II restriction enzymes cleaves the DNA within or near the recognition sequence. These enzymes do not require ATP and are independent from methylase. The type II enzymes are the most useful restriction endonucleases for the daily laboratory work. All our NIPPON Genetics EUROPE restriction enzymes are from type II.

Type III restriction enzymes cleaves DNA about 20 – 25 base pairs away from the recognition sequence. They require both ATP and S-adenosyl-L-methionine to function.

Type IV restriction enzymes cleaves only modified, typically methylated DNA in contrast to the types I-III, which are usually inhibited by methylation.

 

Ambiguity Codes

N = A or C or G or T (any)
B = C or G or T (not A)
D = A or G or T (not C)
H = A or C or T (not G)
V = A or C or G (not T)
W = A or T (weak)
S = C or G (strong)
R = A or G (purine)
Y = C or T (pyrimidine)
M = A or C (amino)
K = G or T (keto)

Dane techniczne

Opakowanie 250 react.

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