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Producenci

FastGene StuI

nr kat.: FG-StuI
Opakowanie: 250 react.
Cena brutto: 200,49 zł 200.49
Cena netto: 163,00 zł
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Producent: Nippon
nr kat.: FG-StuI

Opis

  • No Star Activity
  • Highest Purity
  • Restriction in just 5 minutes with the FastCut buffer
  • Perfect use for Cloning experiments

 

Overhang CpG Methylation
Blunt Not Sensitiv
Incubation Temp. Heat Inactivation
37°C 65°C for 20 min

Activity in FastGene Buffer

Buffer I Buffer II Buffer III Buffer IV FastCut Buffer
75% 100% 75% 100% 100%
Supplied Buffer
Enzyme Type
Buffer IV Type IIP
Isoschizomers
Eco147I, PceI, SseBI
Recognition Sequence
Amount
AGG/CCT
TCC/GGA
1000 units, 4 units/µl

Methylation sensitivity:

dam methylation: Not sensitive

dcm methylation: Conditionally sensitive

CpG methylation: Not sensitive

 

ENZYME FINDER


Different Types of restriction enzymes

Based on the structure, cofactor requirements and specificity of cleavage there are four types of restriction enzymes (Types I, II, III, and IV).

Type I restriction enzymes cleaves the DNA at a random location far away from the recognition sequence. These enzymes require both ATP and S-adenosyl-L-methionine to function.

Type II restriction enzymes cleaves the DNA within or near the recognition sequence. These enzymes do not require ATP and are independent from methylase. The type II enzymes are the most useful restriction endonucleases for the daily laboratory work. All our NIPPON Genetics EUROPE restriction enzymes are from type II.

Type III restriction enzymes cleaves DNA about 20 – 25 base pairs away from the recognition sequence. They require both ATP and S-adenosyl-L-methionine to function.

Type IV restriction enzymes cleaves only modified, typically methylated DNA in contrast to the types I-III, which are usually inhibited by methylation.

 

Ambiguity Codes

N = A or C or G or T (any)
B = C or G or T (not A)
D = A or G or T (not C)
H = A or C or T (not G)
V = A or C or G (not T)
W = A or T (weak)
S = C or G (strong)
R = A or G (purine)
Y = C or T (pyrimidine)
M = A or C (amino)
K = G or T (keto)

Dane techniczne

Opakowanie 250 react.

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