FastGene Western ECL Kit

Chemiluminescent substrate based on luminol for high femtogram or low picogram detection

Key Benefits

• High sensitivity – Detection of antigens with very low concentrations
• Long signal duration – Optimal for digital and film-based imaging
• No optimization required – Easy switch from other brands
• Perfect for PDVF and nitrocellulose membranes
• Compatible with Western Blotting Markers

FastGene^® Western ECL Kit – Chemiluminescent Western Blot Detection

The FastGene^® Western ECL Kit is an enhanced chemiluminescent substrate based on luminol.
It is used to detect horseradish peroxidase (HRP) – conjugated secondary antibodies.
The high femtogram or low picogram detection of antigen is enabled by FastGene^® Western ECL Kit brilliant sensitivity and long signal duration.
This long chemiluminescent signal duration makes it possible to detect signals on both digital and film-based imaging systems without any loss in signal intensity.
Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.

Workflow using FastGene^® Western ECL Kit

Mix the luminol solution and peroxide solution in a 1:1 ratio, and thoroughly agitate the chemiluminescent substrate solution well for preparing the 0.1 ml of solution / cm² of membrane.
Place the membrane with the protein side up and remove the membrane from the chemiluminescent substrate solution. Image the membrane with a digital imager.

Comparison between the FastGene^® Western ECL Kit with 3 competitor products

All ECL kits were used under the same experimental workflow.
An IL-6 fusion protein was detected using a primary antibody against IL-6 from mouse and a secondary anti-mouse peroxide (POD) antibody 2 seconds exposure for the Western Blot.

Contains:

50 ml Peroxide solution

50 ml Luminol Solution