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FastGlycoProtein™ Isolation Kit ConA Resin

nr kat.: 116550800
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Producent: MP Biomedicals
nr kat.: 116550800

Opis

The MP Biomedicals FastGlycoProtein Isolation Kit, isolates glycoproteins from complex protein mixtures including serum and cultured cells as well as animal and plant tissue, microbe and insect lysates using the lectin concanavalin A (ConA) immobilized on agarose.

Lectins are proteins that have a selective affinity for carbohydrate moieties. The ConA lectin preferentially recognizes α-linked mannose and, to a lesser extent, terminal glucose residues. These carbohydrates are attached to proteins through asparagine residues.

Oligomannosyl saccharides are commonly present in a wide variety of serum and membrane-bound glycoproteins.
Protein glycosylation is a common post-translational modification. Asparagine (N-linked) and serine/threonine residues (O-linked) are glycosylated during passage through the endoplasmic reticulum and golgi apparatus in eukaryotic and prokaryotic (i.e., Archaea and Eubacteria) systems. Glycoconjugates are important for immune regulation, inflammation, cell-to-cell adhesion and contact inhibition, cell signaling, protection against proteolytic degradation, and other biological processes.

This kit is easy to use and contains all the necessary components for isolating most N-linked glycans. A sample containing up to 1.5 mg of total protein is first placed in lysing matrix A tube, with the Binding/Wash Buffer applied to it. After the tissue/cell lysis is complete, the sample is transfeered and applied to the ConA resin bed. Following incubation, the resin is washed and the bound glycoproteins are eluted. Glycoproteins have been successfully isolated from serum as well as HeLa and CHO cell lysates in approximately 40 minutes.

The FastPrep Sample Preparation Instruments including the FastPrep-24™ and Fast- Prep-96™ are high-throughput homogenizers developed to disrupt thoroughly any tissue or cells through the simultaneous bead-beating and impaction of specialized Lysing Matrix beads on the sample material. The FastPrep Instruments use high-powered complex motions to provide extremely quick and highly reproducible homogenization that surpasses early generation homogenizers, as well as traditional extraction methods using enzymatic digestion, sonication, blending, douncing or vortexing.

Samples are placed into a variety Lysing Matrix Vessel formats containing custom lysing matrix particles designed for optimized disruption of specific sample types. The lysing matrix particles are designed to efficiently lyse organisms such as plant and animal tissue, cultured cells, bacteria, yeast and fungi, including historically difficult sources such as eubacterial spores and endospores, algae, and nematodes, while in the presence of aspecially formulated lysis solution.

The FastPrep Instruments lyse samples quickly and efficiently, most in 40 seconds or less, all while preserving the protein’s functionality and biological activity.

FastGlycoProtein™ Isolation Kit Components

  • Lysing Matrix A 10 x2 mL Tubes
  • ConA Lectin Resin 1.1 mL settled, as slurry
  • Binding/Wash Buffer. 5X solution 6.5 mL
  • Elution Solution 5 mL
  • Spin Filter with caps 10 each
  • Catch Tubes 20 each

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