FastGlycoProtein™ Isolation Kit WGA Resin
Opis
The MP Biomedicals FastGlycoProtein Isolation Kit, isolates glycoproteins from complex protein mixtures including serum and cultured cells as well as animal and plant tissue, microbe and insect lysates using the lectin wheat germ agglutinin (WGA) immobilized on agarose. Lectins are proteins that have a selective affinity for carbohydrate moieties.
The WGA lectin preferentially binds N-acetyl glucosamine (GlcNAC) and terminal GlcNAC structures that are commonly present in many serum and membrane glycoproteins. WGA also has affinity for sialic acid.
Protein glycosylation is a common post-translational modification. Asparagine (N-linked) and serine/threonine residues (O-linked) are glycosylated during passage through the endoplasmic reticulum and golgi apparatus in eukaryotic and prokaryotic (i.e., Archaea and Eubacteria) systems. Glycoconjugates are important for immune regulation, inflammation, cell-to-cell adhesion and contact inhibition, cell signaling, protection against proteolytic degradation, and other biological processes.
This kit is easy to use and contains all the necessary components for isolating sialic acid/GlcNAC-containing glycans.. A sample containing up to 1.5 mg of total protein is first placed in lysing matrix A tube, with the Binding/Wash Buffer applied to it. After the tissue/cell lysis is complete, the sample is transferred and applied to the ConA resin bed. Following incubation, the resin is washed and the bound glycoproteins are eluted. Glycoproteins have been successfully isolated from serum as well as HeLa and CHO cell lysates in approximately 40 minutes.
The FastPrep Sample Preparation Instruments including the FastPrep-24™ and Fast- Prep-96™ are high-throughput homogenizers developed to disrupt thoroughly any tissue or cells through the simultaneous bead-beating and impaction of specialized Lysing Matrix beads on the sample material. The FastPrep Instruments use high-powered complex motions to provide extremely quick and highly reproducible homogenization that surpasses early generation homogenizers, as well as traditional extraction methods using enzymatic digestion, sonication, blending, douncing or vortexing.
Samples are placed into a variety Lysing Matrix Vessel formats containing custom lysing matrix particles designed for optimized disruption of specific sample types. The lysing matrix particles are designed to efficiently lyse organisms such as plant and animal tissue, cultured cells, bacteria, yeast and fungi, including historically difficult sources such as eubacterial spores and endospores, algae, and nematodes, while in the presence of a specially formulated lysis solution.
The FastPrep Instruments lyse samples quickly and efficiently, most in 40 seconds or less, all while preserving the protein’s functionality and biological activity.
FastGlycoProtein™ Isolation Kit Components
- Lysing Matrix A 10 x2 mL Tubes
- WGA Lectin Resin 1.1 mL settled, as slurry
- Binding/Wash Buffer. 5X solution 6.5 mL
- Elution Solution 5 mL
- Spin Filter with caps 10 each
- Catch Tubes 20 each
Dane techniczne
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