Produkty
Producenci
GENE`s Protein Ladder Extended prestained
Opis
Protein Marker EXtended contains 13 pre-stained proteins that resolve into sharp, tight bands in the range of 3,5-245 kDa.Protein Marker 5-245kD Extended The Protein Ladder allows to monitor molecular weight separation during electrophoresis, estimate molecular weights. The marker is ready-to-use. There is no need to boil. Easy to identify: Proteins are covalently coupled with a blue chromophore except for two reference bands; green band at 25 kDa and red band at 75 kDa.
Applications
Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.
Monitoring of protein transfer onto membranes during Western blotting.
Sizing of proteins on SDS-polyacrylamide gels and Western blots.
Usage:
Mini gel application: 5 μl/well; 2.5 µl per well blots
Standard gel application: 10 μl/well; 5 µl per well for blots
Number of bands: 13 (5, 11, 27, 20, 25, 35, 48, 63, 75, 100, 135, 180, 245 kDa)
Loading:
Loading Denaturing Polyacrylamide gels (SDS-PAGE):
- Thaw marker at room temperature or heat at 37 – 40 °C for a few minutes. Do not boil!
- Vortex gently to ensure the solution is homogeneous and load the ladder on SDS-polyacrylamide gel
- 5 μl per well for mini-gels, 2.5 μl per well for blots
- 10 μl per well for large gels, 5 μl per well for blots
1 ml marker is sufficient for 200 mini gels or 100 standard gels.
It is recommended to divide the marker into aliquots to avoid contamination of the stock solution.
Note:
Protein Marker PS11 is optimized for runs on 15 % SDS polyacrylamide gels. 4 to 12 % gels may cause
proteins with low molecular weights to migrate with the dye front. On 12 to 15 % and gradient gels all bands are visible.
Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.
Storage: at -20°C or at room temperature
Dane techniczne
| Opakowanie | 5x 500µl |
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