GeneJET Stabilized and Fresh Whole Blood RNA Kit

The GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).

Highlights
•  Versatile – compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus ™ Tubes
•  Convenient – specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 h
•  Efficient – up to 4 µg of total RNA from 500 µL of healthy human blood
•  Pure – isolates intact total RNA free of any RT-qPCR inhibitors

Applications

Isolated total RNA can be used in:
•   RT-PCR
•   RT-qPCR
•   Northern Blotting

Includes
•   Lysis Buffer
•   Wash Buffer 1 (conc.)
•   Wash Buffer 2 (conc.)
•   DNase l (lyophilized)
•   DNase I Reconstitution Buffer
•   2x DNase I Buffer
•   Manganese Chloride Solution
•   Water, nuclease-free
•   GeneJET RNA Purification Columns pre-assembled with Collection Tubes
•   Collection Tubes, 2 mL
•   Collection Tubes, 1.5 mL