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HS-Nuclease, recombinant Endonuclease (encoded by the same gene as Benzonase®)

Cena brutto: 1 264,38 zł
Cena netto: 1 170,72 zł
szt.
Opakowanie:
50000 U
Kod produktu:

GE-NUC10700-01

Producent:
Pliki do pobrania:

200 μl HS-Nuclease (250 units/μl) in 50 mM Tris-HCI, 50 mM NaCI, 5 mM MgCI2, 50% Glycerol, pH 8.0

Purity

  • HS-Nuclease is purified through a proprietary process that achieves purity of >99% as shown in Figure 1.
  • Total endotoxin level is <0.25 EU/1,000 units of HS-Nucleaseas determined by the LAL Gel-Clot Assay.
  • HS-Nuclease shows no detectable protease activity.

 

HS-Nuclease is a recombinant form of Serratia marcescens extracellular endonuclease (encoded by the same gene as Bezonase®) produced in E. coli using a proprietary process. This nonspecific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1 - 4 bases in length. HS-Nuclease is a highly purified homodimer of 27 kDa subunits that has exceptional high specific activity and is free of protease activity. HS-Nuclease is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.

Applications
HS-Nuclease can be used to reduce viscosity of cell lysates and remove nucleic acid contaminations from sample preparations. It reduces smearing when used with 10% SDS to make whole cell lysate for SDS-PAGE. It may reduce or prevent clumping of concentrated cells and frozen cells following thawing. HS-Nuclease also replaces crude DNase I in many applications.
To reduce viscosity of cell lysates, 10 - 1000 units of HS-Nuclease can be used per gram of cell paste. The efficiency of viscosity reduction may vary with buffers, cell types, and cell lysis methods used. Due to its high specificity, the total amount of HS-Nuclease added is less than 1 μg/ml of lysate and will not interfere with any downstream processes not involving nucleic acids.
Note: HS-Nuclease is stable in storage buffer at 37 °C for at least three weeks without any loss of activity.

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