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KRAS Q61L Reference Standard, 50%

nr kat.: HD140
Cena brutto: do wyceny
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Opis

Synonyms: C-Ki-Ras, K-RAS2B, KI-RAS, KRAS2, RASK2

Format: FFPE

Description:
The KRAS Q61L Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of assays that detect somatic mutations, such as Sanger and qPCR sequencing assays.

With this product you are able to:

• Evaluate of workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics

• Analyze the sensitivity of your workflow

• Optimize and validate new cancer panels and routinely monitor the performance of your assay

Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 50%, and may be diluted using the corresponding wild type standard to generate a lower allelic frequencies for limit of detection studies and validation of your pre-analytical phase of the workflow.

Horizon’s Base-Seq products are all derived from human cell lines. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly-reproducible and consistent FFPE curls. With any commercially available FFPE extraction protocol, our standards yield high molecular weight DNA. This format ensures that they may applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.

Technical Data
DNA Base Change: CAA→CTA

NCBI Reference Assembly SNP: rs121913240

Cosmic ID: COSM553

Fixation Method:  4% Formalin

Section Size: 15µm or 20µm

Cell Density:  3 x 108 cells per block. Approx. 3.5 x 105 cells per section

Expected DNA Yield:  ≥ 400 ng using Promega Maxwell LEV Plus Extraction kit

Product Information

Intended Use:  For routine performance monitoring (Research Use Only)

Unit Size: 1 FFPE Section per vial

Extractable DNA:  ≥ 400 ng per section using Promega Maxwell LEV Plus FFPE DNA Extraction kit

General Information

Allelic Frequency: 50%

Storage: 4˚C

Expiry: 48 months from the date of manufacture

Cell Line Background: SW48

Quality Control
Allelic Frequency: Droplet Digital PCR™
Genotype: Sanger sequencing of locus specific PCR
Quality: Agarose gel electrophoresis
Quantification: Quantifluor™
Genotype: KRAS (Q61L/+)

Characterization

Chromatogram


Chromatograph showing heterozygosity for the K-Ras Q61L mutation within K-Ras exon 4 (SNP accession number: rs121913529)

Primers
    Primer         Sequence                                        Size / bp
    Forward    TGTTTTCCACAATGGCATCA                  633
    Reverse    TGCATGGCATTAGCAAAGAC
    Sequencing    TGTTTTCCACAATGGCATCA           --

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