Kex2 Protease (Lys/Arg-Arg), recombinant
Opis
- Specific serine endoproteinase
- Cleaves amino acid sequence N-Arg-Arg/-C and N-Lys-Arg/-C at the carboxyl end
- Suited for protein sequencing and cleavage of fusion proteins with an appropriate recognition sequence
In the series of endoproteinases we also offer the newly developed endoproteinase Lys/Arg-Arg. The specific serine endoproteinase (universal serine-protease) has a MW of 68 kDa. It cleaves at the carboxyl end of the recognition sequences: Arg-Arg/X and Lys-Arg/X and thus provides new possibilities for e.g. the development of fusion protein systems with alternative cleavage sites.
Technical Details
Source: Saccharomyces cerevisiae
Activators: Endoproteinase Lys/Arg-Arg is strongly Ca2+dependent and has a pH-optimum at pH 7.0
Inhibitors: Ala-Lys-Arg-chloro-methyl ketone
Note: Endoproteinase Lys/Arg-Arg isnot inhibited by phenyl-methyl-sulfonyl-fluoride and tosyl-lysine-chloro-methylketone.
Reaction buffer: 50 mM Tris-HCl or HEPES, 5 mM CaCl2, pH 7.0 (0.5 mM PMSF; 0.1%Triton X-100 is not included, but might be added if required by yourapplication). For fusion proteins the reaction conditions have to bedetermined empirically.
Substrate solution: Benzyloxycarbonyl-L-tyrosyl-L-lysyl-L-arginin-4-nitroanilid (Z-L-Tyr-Lys-Arg-pNA ◦ TFA salt, Bachem No. L1250). The substrate is dissolved in reaction buffer (OD315= 12). This solution can be stored at -20°C for several months. Thaw at room temperature immediately before use.
Unit definition: 1 unit endoproteinase Lys/Arg-Arg releases 1 μmole 4-nitroaniline perminute in reaction buffer at pH 7.0 at RT.
Specific activity: 2.2 U/ml
Dane techniczne
Opakowanie | 50 U |