Minute™ Total Protein Extraction Kit  for Animal Cultured Cells/Tissues

 
Invent Biotechnologies Minute^TM total protein extraction kit for animal cultured cells and tissues is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml collection tubes.
The kit is designed to rapidly extract total proteins from animal cells and tissues (invertebrate and ve
rtebrate) for protein analysis and further purification. Since protein profiles extracted by denaturing and native cell lysis buffer are not identical, for a given application one lysis buffer might be superior to the other. This kit provides both denaturing and native cell lysis buffers for users to test and select the best one for a specific application. Due to the use of the protein extraction filter cartridges, the extraction volume can be as small as 20 μl and as large as 500 ul. This unique feature is very useful in situations where available starting material is a limiting factor. Total proteins can be extracted from cultured cells/tissues in 1-8 min with high yield (2-8 mg/ml).

Application
Minute^TM total protein extraction kit is designed to rapidly extract total proteins from
invertebrate and vertebrate cultured cells and tissues for applications such as SDS-PAGE, immunoblottings, IP, ELISA, enzyme assays and other applications. This kit provides the most rapid method currently available for preparation of whole cell protein extract. Extracted proteins can also be use as a good starting material for small scale protein purification in column chromatography.

Buffer Formulation: Proprietary

Kit components
1. 25 ml denaturing cell lysis buffer (SD-001)
2. 25 ml Native cell lysis buffer (SN-002)
3. 50 protein extraction filter cartridges
4. 50 collection tubes with cap
5. Plastic rods (4)

Protein profiles of animal tissues extracted by denaturing lysis buffer and native lysis buffer.
Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1: drosophila larvae; Lane 2: drosophila pupa; Lane 3: adult drosophila; Lane 4: gold fish muscle; Lane 5: mouse liver

Important Product Information
The Minute^TM total protein extraction kits are designed to extract total protein rapidly. The use of protease inhibitors is not necessary prior to extraction. However if downstream
application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease in hibitors to cell lysis buffer is recommended.
For determination of protein concentration, BCA kit  is recommended. To study protein phosphorylation,
phosphatase inhibitors should be added to lysis buffer prior to use.

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