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MoBiTec Ni-IDA Columns

nr kat.: PR-HTK004
Opakowanie: 4 szt./op.
Cena brutto: do wyceny
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Producent: MoBiTec
nr kat.: PR-HTK004

Opis

 

  • Excellent tool for routine purification of recombinant polyhistidine-tagged proteins
  • Purification under native and denaturating conditions
  • Starting from diverse expression systems, e.g., E. coli, yeast, insect, and mammalian cells
  • Maximal binding capacity: 90 mg protein per column
  • Protein recovery rate > 80%
  • Improved target specificity by optimized silica-based Ni2+-IDA matrix
  • Imidazol free loading and washing buffer
  • Columns are long-term storable when kept dry

 


Description
MoBiTec Ni-IDA Columns with 1 g silica-based resin provide a fast and convenient routine tool for purification of recombinant polyhistidine-tagged proteins by gravity flow.

The form-stable silica matrix is precharged with Ni2+ ions and allows purification on the principle of Immobilized Metal Ion Affinity Chromatography (IMAC). Binding of proteins is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions. The chelating group of the Ni-IDA resin is based on IDA (iminodiacetic acid), which enables strong and efficient binding of target protein onto the IMAC matrix.

In contrast to traditional IDA matrices, MoBiTec Ni-IDA is an optimized matrix with low density of IDA ligands. This non-saturating surface concentration of IDA eliminates almost all non-specific interactions of contaminating host proteins with the adsorbent. As a result, MoBiTec Ni-IDA provides higher target protein purity.

IDA is a tridentate chelator which occupies three of the six binding sites in the coordination sphere of the Ni2+ ion. The remaining three coordination sites are usually occupied by water molecules and can be exchanged with histidine residues of the recombinant protein.

MoBiTec Ni-IDA Columns
Equilibrated with Lysis-Equilibration-Wash Buffer (left) and with bound 6xHis-GFP (right)

Dane techniczne

Opakowanie 4 szt./op.

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