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MobiTEVProtease1 (HighSpeed), recombinant, His-Tag

Cena brutto: 621,27 zł
Cena netto: 505,10 zł
szt.
Opakowanie:
1000 U
Kod produktu:

PR-ETA10010-01

Producent:
Pliki do pobrania:

Description

MobiTEV  Protease1  (HighSpeed)  is  used  to  remove  fusion  tags -solubility,  secretion,  detection,  and purification tags -and to release the "native" protein of interest. MobiTEV Protease1 (short: MobiTEV1) is an  improved  version  of  Tobacco  Etch  Virus  (TEV)  protease  that  is  highly  site-specific,  highly  active,  and significantly  more  stable  than  native  TEV  Protease,  resulting  in  enhanced  long-term  activity. 

MobiTEV1 specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly). The MobiTEV1, is fused to a 6xHis-Tag and can be removed from the reaction mixture by Ni-IDA or Ni-NTA chromatography.

Source

The DNA sequence encoding a mutant form of nuclear inclusion protein a (NIa) protease from tobacco etch virus  (TEV  Protease)  was  fused  to  a  hexahistidine  tag.  To  avoid  proteolytic  auto-inactivation,  the  serine amino acid residue at position 219 was replaced by valine.The chimeric protein was expressed in E. coliBL21(DE3) and S219V TEV Protease mutant was purified on a Ni-NTA column.

 

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