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Producenci

MulV First-Strand cDNA Synthesis Kit

nr kat.: MB17201
Opakowanie: 50 react.
Cena brutto: 566,78 zł 566.78
Cena netto: 460,80 zł
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Producent: NZYTech
nr kat.: MB17201

Opis

 NZY M-MuLV First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA. The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY M-MuLV First-Strand cDNA Synthesis Kit is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg up to 5 µg of total RNA.

The kit includes a combination of random hexamers and oligo(dT)18 primers in order to increase sensitivity. The primers are included in the NZYRT 2× Master Mix, which also contains dNTPs, MgCl2 and an optimized RT buffer. NZYM-MuLV RT Enzyme Mix includes both NZY M-MuLV Reverse Transcriptase (RNase H minus) and NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. RNase H (from E. coli) is provided in a separate tube to specifically degrade the RNA template in cDNA:RNA hybrids after the first-strand cDNA synthesis. This procedure will improve the sensitivity of subsequent RT-qPCR reaction since PCR primers will bind more easily to the cDNA.

Features:
– Cost-effective kit using NZY M-MuLV Reverse Transcriptase
– Provides high yields of full-length cDNA products
– Formulated to increase sensitivity in RT-qPCR
– Primer type: oligo(dT)18 and random hexamers
– Starting material: 10 pg to 5 µg of total RNA
– Optimal reaction temperature: 37 °C
– Convenient and reliable

Applications:
– First-strand cDNA synthesis for use in real-time quantitative RT-PCR (RT-qPCR)
– Two-step RT-PCR assays

Specifications:
– Storage conditions: Store all kit components at -20ºC
– Shipping conditions: Shipped at 4 ºC to dry ice

Components:
– NZYM-MuLV RT Enzyme Mix
– NZYRT 2x Master Mix
– NZY RNase H (E. coli)
– DEPC-treated H2O

Dane techniczne

Opakowanie 50 react.

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