Multiplex I cfDNA Reference Standard Set in Synthetic Matrix

Format: cfDNA

Developed using clinically-relevant mutations in an artificial matrix to give you full control of your cfDNA workflow, reducing the likelihood of a false negative or positive due to incorrect assay loading. Multiplex I cfDNA Reference Standard Set in synthetic matrix contains 8 onco-relevant mutations to measure your extraction efficiency. Put the control of your workflow back in your hands.

The Multiplex I cfDNA Reference Standard Set in artificial matrix covers multiple engineered single nucleotide variants (SNVs/SNPs) with 8 mutations at 5%, 1%, and 0.1% allelic frequencies.

For information on specific gene copies detected in each product batch, download the product insert from the Protocols and Documents tab.

Technical Data

Genes Covered: EGFR, KRAS, NRAS, PI3KCA

Cosmics: 1,500+

Allelic Frequencies: 5%, 1%, 0.1% and 0% (100% wild type)

Buffer: Synthetic matrix

Expected DNA Yield: ≥120 ng DNA using the QIAamp® Circulating Nucleic Acid Kit

Product Information

Fragment Size: 170bp

Unit Size: 400ng per 1ml (4 vials total)

Presence confirmed in parental cell line: EGFR G719S (20% allelic frequency); download additional information on variants present in the parental cell lines:
    





General Information

Storage: 4°C

Expiry: Stable for 24 months from date of manufacture (as supplied)

Cell Line Background: RKO/SW48

Quality Control

Fragmentation Size: D1000 DNA ScreenTape assay
Allelic Frequency: Droplet Digital™ PCR
Quantification: Qubit dsDNA BR Assay (post-fragmentation)