Multiplex I cfDNA Reference Standard Set in Synthetic Plasma
Opis
Format: cfDNA
Developed using clinically-relevant mutations in synthetic plasma to give you full control of your cfDNA workflow, reducing the likelihood of a false negative or positive due to incorrect assay loading. Multiplex I cfDNA Reference Standard Set in synthetic plasma contains 8 onco-relevant mutations to measure your extraction efficiency. Put the control of your workflow back in your hands.
The Multiplex I cfDNA Reference Standard Set in synthetic plasma covers multiple engineered single nucleotide variants (SNVs/SNPs) with 8 mutations at 5%, 1%, and 0.1% allelic frequencies.
For information on specific gene copies detected in each product batch, download the product insert from the Protocols and Documents tab.
Technical Data
Genes Covered: EGFR, KRAS, NRAS, PI3KCA
Cosmics: 1,500+
Allelic Frequencies: 5%, 1%, 0.1% and 0% (100% wild type)
Buffer: Synthetic plasma
Expected DNA Yield: ≥240 ng DNA (from 2 ml of plasma) using the QIAamp® Circulating Nucleic Acid Kit
Product Information: Fragment Size: 170bp
Unit Size: 400ng per 1ml (8 vials total)
Presence confirmed in parental cell line:
EGFR G719S (20% allelic frequency); download additional information on variants present in the parental cell lines:
General Information
Storage: 4°C
Expiry: Stable for 24 months from date of manufacture (as supplied)
Cell Line Background: RKO/SW48
Quality Control
Fragmentation Size: D1000 DNA ScreenTape assay
Allelic Frequency: Droplet Digital™ PCR
Quantification: Qubit dsDNA BR Assay (post-fragmentation)