Multiplex I cfDNA Reference Standard Set

Format: cfDNA

Description
The Multiplex I cfDNA Reference Standards are highly-characterized, biologically-relevant reference materials used to assess the performance of cfDNA assays that detect somatic mutations, including quantitative PCR, next-generation sequencing, and other novel assays.

With this product you are able to:
• Analyze the sensitivity and specificity of your assay
• Gain certainty of the limit of detection and limit of quantification
• Optimize and validate new cancer panels and routinely monitor the performance of your assay

High throughput platforms such as Next generation sequencing (NGS) and quantitative PCR (qPCR) platforms give researchers much greater power when profiling cfDNA samples. However, the use of these presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s cfDNA products are all derived from human cell lines, and are fragmented to an average size of 160 bp to closely resemble cfDNA extracted from human plasma.

This format ensures that they may applied to a wide-range of cfDNA NGS and qPCR assays including high-throughput platforms such as NGS and qPCR give researchers greater power when profiling cfDNA samples. Horizon has developed the cfDNA Reference Standard range to support the development and continued validation of cfDNA assays.

The Multiplex I cfDNA Reference Standard Set covers multiple engineered single nucleotide variants (SNVs/SNPs) with 8 mutations at 5%, 1%, and 0.1% allelic frequencies. Batch-specific copy number values measured via ddPCR are provided with each Set.


Technical Data

Genes Covered: EGFR, KRAS, NRAS, PI3KCA

Cosmics: 1,500+

Allelic Frequencies: 5%, 1%, 0.1% and 0% (100% wild type)

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0

Product Information

Fragment Size: 160 bp

Unit Size: 350 ng per vial

Concentration: 20 ng/µl

Presence confirmed in parental cell line: EGFR G719S (20% allelic frequency);

Download additional information on variants present in the parental cell lines:

General Information

Storage: 4°C

Expiry: 12 months from the date of manufacture

Cell Line Background: RKO/SW48

Quality Control

Fragmentation Size; D1000 DNA ScreenTape assay

Allelic Frequency: Droplet Digital™ PCR

Quantification: Qubit dsDNA BR Assay (post-fragmentation)