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Multiplex PCR NZYProof 2x Green Master Mix

nr kat.: MB33702
Opakowanie: 200 react.
Cena brutto: 856,70 zł 856.70
Cena netto: 696,50 zł
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Producent: NZYTech
nr kat.: MB33702

Opis

Description: Multiplex PCR NZYProofGreen Master Mix is a premixed ready-to-use solution designed for the simultaneous amplification of multiple DNA fragments (up to 15 targets) in a single tube with high accuracy. The Master Mix contains an engineered proofreading DNA polymerase displaying a hot-start-like PCR capacity and high processivity (fast polymerisation), while reducing errors due to its 3´→5´ exonuclease activity. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers, and thus ensuring highly specific and sensitive multiplex PCR amplification. In addition, the master mix contains dNTPs and reaction buffer at optimal concentrations for efficient multiplexing of targets ranging in size from 70 bp to 2.5 kb over a broad range of primer and template concentrations. MgCl2 final concentration is 1.5 mM, allowing the implementation of a comprehensive variety of PCR protocols. Reactions assembled with Multiplex PCR NZYProof 2× Green Master Mix may be directly loaded onto agarose gels, which simplifies the workflow. There are two dyes (blue and yellow) in the mix that allow monitoring the progress of electrophoresis.

Features:
– High performance in multiplexing up to 15 primer pairs
– Direct Gel Loading
– Proofreading activity
– High sensitivity and specificity
– Minimal optimization requirements
– Leaves an A-overhang

Applications:
– Multiplex PCR
– High-Fidelity PCR
– Typing/ Detection
– Generation of products for TA cloning

Specifications:

Product length: 0-3 kb
Hot-start-like capacity: yes
Extension time: 30 sec/kb at 72 °C
3´→5´ activity: yes
Product overhang: blunt
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped at 4 ºC to dry ice

Components:
Multiplex PCR NZYProof 2× Green Master Mix (0.2 U/μL)

Dane techniczne

Opakowanie 200 react.
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