Description: Multiplex PCR NZYTaq 2× Colourless Master Mix is a premixed ready-to-use solution designed for the simultaneous amplification of multiple DNA fragments (up to 15 targets) in a single tube. The Master Mix contains dNTPs and reaction buffer at optimal concentrations for efficient multiplexing of targets ranging in size from 70 bp to 2.5 kb over a broad range of primer and template concentrations. MgCl2 final concentration is 2.5 mM, allowing the implementation of a comprehensive variety of PCR protocols. Amplification efficiency is further improved by the inclusion of a recombinant modified enzyme derived from Taq DNA polymerase, which was engineered to display a hot-start-like PCR capacity and high processivity (fast polymerisation). The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers, and thus ensuring highly specific and sensitive multiplex PCR amplification.
Features:
– High performance in multiplexing up to 15 primer pairs
– High sensitivity and specificity
– Minimal optimization requirements
– Leaves an A-overhang
Applications:
– Multiplex PCR
– PCR
– Typing/ Detection
– Generation of products for TA cloning
Specifications:
| Product length: | 0-3 kb |
| Hot-start-like capacity: | yes |
| Extension time: | 30 sec/kb at 72 °C |
| 3´→5´ activity: | no |
| Product overhang: | 3’-A |
| Storage conditions: | Store at -20 ºC |
| Shipping conditions: | Shipped at 4 ºC to dry ice |
Components:
Multiplex PCR NZYTaq 2× Colourless Master Mix (0.2 U/μL)