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Myeloid DNA Reference Standard

nr kat.: HD829
Cena brutto: do wyceny
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Opis

Format: Genomic DNA

The Myeloid DNA Reference Standard is a well characterised, cell line derived control material manufactured under ISO 13485 that contains 22 variants across 19 genes relevant to myeloid cancer. This exciting new Multigene Multiplex product allows labs to perform quick, more reliable and cost-effective validation of myeloid sequencing assays - saving the user time and money to achieve their quality assurance goals faster.

The Myeloid DNA Reference Standard contains a large number of variants within one tube –meaning users only need to handle one control sample in order to validate a large proportion of their targeted sequencing assay at once. This includes 6 insertions/deletions (INDELs), which can help users to optimise their assay’s performance when sequencing challenging structural variants. In particular, due to the importance of FLT3 internal tandem duplications (FLT3-ITDs), which are present in nearly 25% of patients with AML, Horizon has engineered a large novel 300bp insertion into FLT3 – which we have termed the “ITD300” variant. This is a very desirable feature of this new product, as it represents the types of large insertions that may be encountered in real patient samples but are very difficult to source for assay validation and optimisation purposes.

The Myeloid DNA Reference Standard is designed with 15 mutations present at 5% allele frequency, to support the calculation of clinically relevant LODs of somatic workflows. In addition, the product contains 7 mutations at 10-70% allele frequency to support the validation of higher frequency germline workflows.

Technical Data

Genes Covered: ABL1, ASXL1, BCOR, CBL, DNMT3A, EZH2, FLT3, GATA1, GATA2, IDH1, IDH2, JAK2, KRAS NPM1, NRAS, RUNX1, SF3B1, TET2, TP53

Cosmics: 20

Allelic Frequencies: 5 - 70%

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.1

Unit Size: 500ng

Concentration: 25ng/µl

General Information

Storage: 4˚C

Expiry:  12 months from the date of manufacture

Quality Control

Allelic Frequency: Droplet Digital PCR™

Genotype: Sanger sequencing of locus specific PCR

Quality: Agarose gel electrophoresis

Quantification:  Spectrophotometry (A260)

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