NZY M-MuLV Reverse Transcriptase

NZY M-MuLV Reverse Transcriptase is a recombinant form of the Reverse Transcriptase from the Moloney Murine Leukemia Virus (M-MuLV) purified from Escherichia coli. The enzyme synthesizes the complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. NZY M-MuLV Reverse Transcriptase lacks 3’→5´ exonuclease activity and has no RNase H activity thus enabling improved synthesis of full-length cDNA, even for long mRNA, using random priming.

Features:

• Synthesizes cDNA from RNA or ssDNA
• High yields of first-strand cDNA
• No intrinsic RNase H activity

Applications:

• First-strand cDNA synthesis for RT-PCR and RT-qPCR
• Synthesis of cDNA for cloning and expression
• Analysis of RNA by primer extension

Specifications:

• Product length: up to 7 kb
• Sensitivity:  high
• Optimal reaction temperature: 37 ºC
• Available as Kits:  NZY M-MuLV First-Strand cDNA Synthesis Kit (MB172); NZY M-MuLV First-Strand cDNA Synthesis Kit, separate oligos (MB173); NZY M-MuLV First-Strand cDNA Synthesis Kit, no oligos (MB174)
• Speed: 50 min
• Denaturing conditions: NZY M-MuLV Reverse Transcriptase is inhibited in the presence of metal chelators (e.g. EDTA), inorganic phosphate, pyrophosphate and polyamines. The enzyme is inactivated at 70 °C for 15 min.
• Storage conditions: Store at -20 ºC
• Shipping conditions: Shipped at 4 ºC to dry ice

Components:

• NZY M-MuLV Reverse Transcriptase (200 U/μL)
• Reaction Buffer (10x)

1. NZY First-Strand cDNA Synthesis Kit
2. NZY First-Strand cDNA Synthesis Kit, separate oligos
3. NZY First-Strand cDNA Synthesis Kit, no oligos