NZY Mag Viral RNA/DNA Isolation Kit, IVD

200 preps

NZY Mag Viral RNA/DNA Isolation kit, IVD, is a magnetic bead technology‑based nucleic acid purification kit designed to recover RNA and DNA from viral particles contained in transport medium from human nasopharyngeal swabs. The kit uses magnetic-bead technology to provide reproducible recovery of high-quality nucleic acids. The procedure is based on the reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions.

Sample lysis and binding to the NZY Mag Binding Beads is achieved by incubation with NZY Mag Binding Buffer containing chaotropic ions supported by nucleic acid release with NZY Mag Proteinase K digestion. Following nucleic acid magnetic separation, paramagnetic beads holding the RNA/DNA are washed to remove contaminants and salts using NZY Mag Wash Buffer and 80% ethanol. Residual ethanol from previous wash steps is removed by air-drying. Finally, highly pure viral RNA/DNA is eluted with NZY Mag Elution Buffer. It is highly recommended to use extraction controls, positive and negative controls, internal controls as well as to perform cross-contamination assays during the usage of NZY Mag Viral RNA/DNA Isolation kit, IVD.

Protocol options for NZY Mag Viral RNA/DNA Isolation Kit, IVD, support automated workflows in a variety of platforms, including the Thermo Scientific^TM KingFisher^® Flex Magnetic Particle Processor with a 96 deep-well head. Automated workflows allow for 96 nasopharyngeal swab specimens to be processed in <30 minutes. In addition, a flexible protocol accommodates sample volume inputs of 200 μL of transport medium with an elution volume of 50 μL. High-quality nucleic acids are subsequently ready to use for detection using different amplification strategies, such as Reverse Transcription (RT)-PCR or RT-qPCR and quantitative PCR (qPCR). NZY Mag Viral RNA/DNA Isolation kit, IVD, is intended for use by professional users experienced and trained in molecular biology techniques, including experience with swabs and potentially infectious human sample materials.

Features:

• High viral RNA/DNA quality
• Viral RNA from human nasopharyngeal swabs
• High-speed protocol (< 30 minutes)
• Magnetic bead technology
• Easy automation – compatible with automated magnetic separators, including Thermo Scientific^TM KingFisher^® Flex Magnetic Particle Processor

Applications:

The purified RNA/DNA is suitable for use in the most demanding molecular biology applications, including:

• Reverse transcriptase PCR (RT-PCR)
• Real-time quantitative PCR (qPCR)
• cDNA synthesis

Specifications:

• Sample material (general): human nasopharyngeal swabs
• Format: magnetic beads
• Sample amount: 200 μL of transport medium from human respiratory swabs
• Elution volume: 50 μL
• Preparation time: < 30 min
• High-throughput compatibility: Compatible with Thermo Scientific^TM KingFisher^® Flex Magnetic Particle Processor
• Product size: 200 preps (MD04881) and 2000 preps (MD04882)
• Storage conditions: room temperature
• Shipping conditions: room temperature

Components:

• NZY Mag Binding Buffer
• NZY Mag Proteinase K
• NZY Mag Wash Buffer
• NZY Mag Elution Buffer
• NZY Mag Binding Beads