Description: NZY Plant/Fungi gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from plant tissues and fungi samples. The method is spin column silica-based and requires no phenol or chloroform extraction. This kit uses two optimized lysis buffers systems based on the established CTAB and SDS methods. NZY Homogenization columns are included in this kit for conveniently clearing the crude lysates. The clear flow-through is mixed with Binding buffer PN for optimal binding of DNA to the silica membrane. Then, the DNA is selectively absorbed into the NZYSpin Plant Column and others impurities such as proteins and salts are removed during the washing steps.
The eluted genomic DNA has a A260/280 ratio between 1.7 and 1.9 what makes it ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.
Features:
– Two optimized lysis buffers
– NZYHomogenezation columns included for clarification of lysates
– High genomic DNA quality
– High yields from plant and fungi samples
– Fast and easy small-scale protocol
– Silica-membrane technology
– RNase A included
Applications:
The purified genomic DNA is suitable for use in the most demanding molecular biology applications, including:
– PCR
– Real time quantitative-PCR (qPCR)
– Genotyping
– DNA sequencing
– Multiplex-PCR
– Enzimatic manipulations
Specifications:
Sample material: | Plant/Fungal tissues |
Format: | Spin-column |
Sample amount: | < 100 mg plant tissue (wet weight)/ < 20 mg plant tissue (dry weight) |
Typical yield | up to 30 μg |
A260/A280: | 1.7-1.9 |
Elution volume: | 50-100 μL |
Binding capacity: | 60 μg |
Preparation time: | 30 min |
High-throughput compatibility: | No |
Product size: | 50 and 200 columns |
Storage conditions: | Store all components at room temperature |
Shipping conditions: | Shipped at room temperature |
Components:
– Buffer PNL1
– Buffer PNL2
– Buffer PNL3
– Buffer PN
– Buffer PNW1
– Buffer PNW2 (concentrated)
– Buffer PNE
– RNase A(lyophilized)
– NZYHomogenization columns
– NZYSpin Plant columns
– Collection tubes