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NZY Ribonuclease Inhibitor (2500u)

Cena brutto: 268,88 zł
Cena netto: 218,60 zł
2500 U
Kod produktu:


Pliki do pobrania:

NZY Ribonuclease Inhibitor is a recombinant protein purified from Escherichia coli that inhibits the activity of ribonucleases (RNases; EC 3.1) of the pancreatic type. The protein is useful in any application where eukaryotic RNase contamination is a potential problem. It is not active against RNase 1, RNase T1, RNase T2, S1 nuclease and RNase H.

In first-strand cDNA synthesis experiments, 40 units of protein in a 20 µL reaction mixture protect the template RNA, improve total cDNA yields and increase the percentage of full length cDNA.

The presence of NZY Ribonuclease Inhibitor does not affect the use of RNase H after first-strand cDNA synthesis. NZY Ribonuclease Inhibitor requires 0.5 to 1 mM DTT (not provided) in the reaction system to maintain activity. The storage buffer of this protein contains 8 mM DTT, but additional DTT is required if volume of the inhibitor in the reaction mixture is less than 1/8 of the total volume.


  • Inhibitor of RNase A, B and C
  • Protects RNA at temperatures up to 55 ºC


  • Protects RNA from degradation in the following situations:
  • cDNA synthesis by reverse transcription
  • in vitro transcription/ translation
  • RNA amplification
  • RNA purification and storage


  • Optimal reaction temperature: 50 ºC
  • Denaturing conditions: NZY Ribonuclease Inhibitor is inhibited by common denaturants such as SDS, urea and all oxidizing reagents. Temperatures above 65 °C also inactivate this protein
  • Storage conditions: Store at -20 ºC
  • Shipping conditions: Shipped on dry ice

Ribonuclease Inhibitor (40 U/µL)


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