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NZY Ribonuclease Inhibitor (no DTT)

nr kat.: MB41002
Cena brutto: 680,07 zł 680.07
Cena netto: 552,90 zł
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Producent: NZYTech
nr kat.: MB41002

Opis

Description: NZY Ribonuclease Inhibitor (no DTT) is a highly pure recombinant enzyme displaying improved resistance to oxidation, which allows it to be stable at DTT concentrations lower than 1 mM. This property makes this ribonuclease inhibitor ideal for reactions that do not tolerate higher levels of DTT (>1 mM) and were RNases (EC 3.1) contamination is a potential problem, such as in real-time RT-PCR assays.

NZY Ribonuclease Inhibitor (no DTT) inhibits the activity of the omnipresent RNases of the pancreatic type, such as RNase A, RNase B and RNase C, by binding them noncovalently in a 1:1 ratio. It is not active against RNase 1, RNase T1, RNase T2, S1 nuclease and RNase H.

Features:
– Eco-friendly room temperature shipment
– High oxidation resistance (which can be helpful in situations where DTT cannot be used)
– Inhibitor of RNase A, B and C
– Protects RNA at temperatures up to 55 ºC

Applications:
Protects RNA from degradation in the following situations:
– cDNA synthesis by reverse transcription
– RT-PCR
-in vitro transcription/ translation
– RNA labelling
– RNA isolation and purification

Specifications:

Optimal reaction temperature:  50 ºC
Denaturing conditions: NZY Ribonuclease Inhibitor is inhibited by common denaturants such as SDS, urea and all oxidizing reagents. Temperatures above 65 °C also inactivate this protein
Lyo format available: Lyo NZY Ribonuclease Inhibitor (no DTT) (MB412)
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped on dry ice

 

Components:
– Ribonuclease Inhibitor (no DTT) (40 U/µL)

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