Description: NZY Ribonuclease Inhibitor (no DTT) is a highly pure recombinant enzyme displaying improved resistance to oxidation, which allows it to be stable at DTT concentrations lower than 1 mM. This property makes this ribonuclease inhibitor ideal for reactions that do not tolerate higher levels of DTT (>1 mM) and were RNases (EC 3.1) contamination is a potential problem, such as in real-time RT-PCR assays.
NZY Ribonuclease Inhibitor (no DTT) inhibits the activity of the omnipresent RNases of the pancreatic type, such as RNase A, RNase B and RNase C, by binding them noncovalently in a 1:1 ratio. It is not active against RNase 1, RNase T1, RNase T2, S1 nuclease and RNase H.
Features:
– Eco-friendly room temperature shipment
– High oxidation resistance (which can be helpful in situations where DTT cannot be used)
– Inhibitor of RNase A, B and C
– Protects RNA at temperatures up to 55 ºC
Applications:
Protects RNA from degradation in the following situations:
– cDNA synthesis by reverse transcription
– RT-PCR
-in vitro transcription/ translation
– RNA labelling
– RNA isolation and purification
Specifications:
Optimal reaction temperature: | 50 ºC |
Denaturing conditions: | NZY Ribonuclease Inhibitor is inhibited by common denaturants such as SDS, urea and all oxidizing reagents. Temperatures above 65 °C also inactivate this protein |
Lyo format available: | Lyo NZY Ribonuclease Inhibitor (no DTT) (MB412) |
Storage conditions: | Store at -20 ºC |
Shipping conditions: | Shipped on dry ice |
Components:
– Ribonuclease Inhibitor (no DTT) (40 U/µL)